Dr. Tsutomu TSUCHIDA - PI
Associate Professor

Graduate School of
Science and Engineering
University of Toyama

3190 Gofuku, Toyama, Toyama 930-8555, JAPAN

Japanese / English

Insect-Microbe Symbiosis

Many insects possess microorganisms that have efficient metabolic functions in their bodies. These symbiotic microorganisms have crucial roles in survival, reproduction, environmental adaptation, and evolution of the host insects. We are aiming to clarify molecular mechanisms underlying symbiotic associations between insects and microorganisms. We intend to establish novel selective inhibition methods for understanding the molecular functions, and to apply the methods to insect pest control.

Selected Publications

* Corresponding author

30) Morioka E, Oida M, Tsuchida T, Ikeda M. "Nighttime activities and peripheral clock oscillations depend on Wolbachia endosymbionts in flies", Scientific Reports 8 : 15432 (2018)

29) Nikoh N, Tsuchida T, Maeda T, Yamaguchi K, Shigenobu S, Koga R, Fukatsu T. "Genomic insight into symbiosis-induced insect color change by a facultative endosymbiont “Candidatus Rickettsiella viridis”", mBio 9 : e00890 – 18 (2018)

28) Eclair M, Polin S, Jousseaume T, Simon JC, Sugio A, Morliè S, Fukatsu T, Tsuchida T, Outreman Y. "Consequences of coinfection with protective symbionts on the host phenotype and symbiont titres in the pea aphid system", Insect Science 24: 798-808 (2017)

27) Tsuchida T*. "Molecular basis and ecological relevance of aphid body colors", Current Opinion in Insect Science 17: 74-80 (2016)

26) De Clerck C, Fujiwara A, Joncour P, Léonard S, Félix ML, Francis F, Jijakli MH, Tsuchida T, Massart S. "A metagenomic approach from aphid’s hemolymph sheds light on the potential roles of co-existing endosymbionts", Microbiome 3: 63 (2015)

25) Polin S, LeGallic J-F, Simon J-Ch, Tsuchida T, Outreman Y. “Conditional reduction of predation risk provided by a facultative symbiont to its insect host”, PLoS One 10: e0143728 (2015)

24) Kurata A, Fujiwara A, Haruyama N, Tsuchida T*. “Multiplex PCR method for rapid identification of genetic group and symbiont infection status in Bemisia tabaci (Hemiptera: Aleyrodidae)”, Applied Entomology and Zoology (in press)

23) Sugimoto TN, Kayukawa T, Ishikawa Y, Tsuchida T. "Misdirection of dosage compensation underlies bidirectional sex-specific death in Wolbachia-infected Ostrinia scapulalis”, Insect Biochemistry and Molecular Biology 66: 72-76 (2015)

22) Sugimoto TN, Kayukawa T, Matsuo T, Tsuchida T, Ishikawa Y. "A short, high-temperature treatment of host larvae to analyze Wolbachia-host interactions in the moth Ostrinia sacpulalis", Journal of Insect Physiology81: 48-51 (2015)

21) Sugimoto TN, Tsuchida T*. “Simple electroporation device for gene functional analyses in insects” Applied Entomology and Zoology, 50: 271-275 (2015)

20) Fujiwara A, Maekawa K, Tsuchida T*. “Genetic groups and endosymbiotic microbiota of the Bemisia tabaci species complex in Japanese agricultural sites” Journal of Applied Entomology, 139: 55-66 (2015)

19) De Clerck C., Tsuchida T., Massart S., Lepoivre P., Francis F., Jijakli MH. "Combination of Genomic and Proteomic Approaches to Characterize the Symbiotic Population of the Banana Aphid (Hemiptera: Aphididae)" Environmental Entomology, 43: 29-36 (2014)

18) Tsuchida T*., Koga R., Fujiwara A., Fukatsu T*. "Phenotypic effect of Candidatus Rickettsiella viridis", a facultative symbiont of the pea aphid (Acyrthosiphon pisum), and its interaction with a coexisting symbiont" Applied and Environmental Microbiology, 80: 525-533 (2014)Cover of the issue

17) Katayama N., Tsuchida T., Hojo MK., Ohgushi T. "Aphid Genotype Determines Intensity of Ant Attendance: Do. Endosymbionts and Honeydew Composition Matter?Annals of the Entomological Society of America 106(6): 761-770 (2013)

16) Masuoka Y., Miyazaki S, Saiki R, Tsuchida T, Maekawa K.“High Laccase2 expression is likely involved in the formation of specific cuticular structures during soldier differentiation of the termite Reticulitermes speratus Arthropod Structure & Development 42: 469-475 (2013)

15) Koga R., Meng X.-Y., Tsuchida T., Fukatsu T. "Cellular mechanism for selective vertical transmission of an obligate insect symbiont at the bacteriocyte–embryo interface" Proceedings of the National Academy of Sciences. 109: 7597-7598 (2012)

14) Simon JCh., Boutin S., Tsuchida T., Koga R., Le Gallic J., Frantz A, Outreman Y., Fukatsu T. “Facultative symbiont infections affect aphid reproduction” PLoS ONE. 6: e21831 (2011)

13) Tsuchida T. Koga R., Matsumoto S., Fukatsu T. “Interspecific symbiont transfection confers a novel ecological trait to the recipient insect” Biology Letters. 7: 245-248 (2011)

12) Tsuchida T*. Koga R., Horikawa, M., Tsunoda, T., Maoka, T., Matsumoto, S., Simon, J.-C., Fukatsu T*. “Symbiotic bacterium modifies aphid body color” Science. 330: 1102-1104 (2010)

11) Koga R., Tsuchida T., Fukatsu T. “Quenching autofluorescence of insect tissues for In situ detection of endosymbionts.” Applied Entomology and Zoology, 44: 281-291 (2009)

10) Tsuchida, T., Koga, R., Fukatsu, T. “Endosymbiont that broadens food plant range of host insect”: Chapter 6 in Insect Symbiosis Volume 3 (Ed. by K. Bourtzis and T.Miller), CRC Press, Boca Raton. 123-141.(2008)

9) Simon J.-C., Sakurai, M., Bonhomme J., Tsuchida, T., Koga, R., Fukatsu, T. “Elimination of a specialized facultative symbiont does not affect the reproductive mode of its aphid host.” Ecological Entomology 32, 296-301. (2007)

8) Koga, R., Tsuchida, T., Sakurai, M., Fukatsu, T. “Selective elimination of aphid endosymbionts: effects of antibiotic dose and host genotype, and fitness consequences.” FEMS microbiology Ecology 60, 229-239. (2007)

7) Tsuchida, T., Koga, R., Sakurai, M., Fukatsu, T. “Facultative bacterial endosymbionts of three aphid species Aphis craccivora, Megoura crassicauda and Acyrthosiphon pisum, sympatrically found on the same host plants.” Applied Entomology and Zoology 41, 129-137. (2006)

6) Sakurai, M., Koga, R., Tsuchida, T., Meng, X.Y., Fukatsu, T. “Rickettsia symbiont in the pea aphid Acyrthosiphon pisum: Novel cellular tropism, effect on host host fitness, and interaction with the essential symbiont Buchnera.” Applied and Environmental Microbiology 71, 4069-4075. (2005)

5) Tsuchida, T., Koga, R., Meng, X.Y., Matsumoto, T., Fukatsu, T. “Characterization of a facultative endosymbiotic bacterium from the pea aphid Acyrthosiphon pisum.” Microbial Ecology 49, 126-133. (2005)This article was selected by faculty of 1000 biology.

4) Tsuchida, T., Koga, R., Fukatsu, T. “Host Plant Specialization Governed by Facultative Symbiont.” Science 303, 1989. (2004)

3) Koga R., Tsuchida T., Fukatsu T. “Changing partners in an obligate symbiosis: a facultative endosymbiont can compensate for loss of the essential endosymbiont Buchnera in an aphid.” Proceedings of the Royal Society of London Series B, 270: 2543-2550 (2003)

2) Tsuchida T., Koga R., shibao H., Matsumoto T., Fukatsu T. “Diversity and geographic distribution of secondary endosymbiotic bacteria in natural population of the pea aphid, Acyrthosiphon pisum.” Molecular Ecology 11: 2123-2135. (2002)

1) Fukatsu T., Tsuchida T., Nikoh N., Koga R. “Spiroplasma symbiont of the pea aphid, Acyrthosiphon pisum (Insecta: Homoptera).” Applied and Environmental Microbiology 67: 1284-1291. (2001)

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